Extracellular Vesicle (EV) are important for the cellular communication in any organism and will be used in molecular diagnostics.

The EV workshop will give you a first introduction into EVs and explain the MISEV guidelines.

Furthermore, various EV isolation methods and EV characterisation methods (e.g. NTA) will be presented.

A focus will be on EV protein and EV RNA biomarkers, how to find and quantify these, e.g. via NGS, PCR and bioinformatical tools.

  • Introduction & Moderation & MISEV guidelines - Michael Pfaffl, TUM, School of Life Sciences, Freising, Germany
  • Extracellular Vesicles – How to find the needle in the haystack - Christian Preusser, EV Core Facility, Medical Faculty, University Marburg, Germany
  • EV Isolation & RNA Isolation
  • Coffee Break
  • EV Characterization of NTA - Fluorescence Colocalisation Measurements - Christina Klasen, Particle Matrix R&D, Inning am Ammersee, Germany
  • EV Biomarker Discovery   (isolation method comparison, small-RNA Seq and qPCR validation) - Michael Pfaffl, TUM, School of Life Sciences, Freising, Germany
  • Biomarker Quantification   (e.g. via dPCR & qPCR)
  • caRNAge -- comprehensive small RNA Seq data analysis - Benedikt Kirchner, TUM, School of Life Sciences, Freising, Germany
  • Final Q&A and Discussion