Target audience: Researchers and clinicians at all levels interested in molecular methods for biomarker analyses

Entrance qualifications: Basic knowledge of molecular methods

This course by Mikael Kubista will present methods for nucleic acid and protein analyses covering expression profiling, microRNA profiling, protein profiling, rare sequence variant analysis, methylation analysis, copy number variation analysis and analysis of results including optimization of workflows and quality control. Relevant guidelines and recommendations are discussed, The course is lecture based.

  • Background and history
  • qPCR – how to plan, optimize and validate
  • dPCR – how to plan, optimize and validate
  • NGS - how to plan, optimize and validate
  • Protein analysis with NGS (profiling) and dPCR (ultrasensitive targeted)
  • Preanalytics – How to identify the confounding step and how to optimize
  • The MIQE and dMIQE guidelines, ISO  guidelines
  • Discussion and Q&A