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Dr Kubista is the head of the department of gene expression at the institute of Biotechnology of the Czech Academy of Sciences, founder and CEO of the TATAA Biocenter (www.tataa.com) and founder of Life Genomics (www.lifegenomics.se). He was one of the pioneers contributing to the development of quantitative real-time PCR (qPCR) and introduced qPCR for single cell expression profiling. He led the development of reagents for high throughput single cell expression profiling and quality control at TATAA. He also developed qPCR tomography for intracellular expression profiling. Kubista coauthored the Minimum Information for Publication of Quantitative. Real-Time PCR Experiments (MIQE) guidelines and is member of the CEN and ISO workgroups drafting the guidelines on preanalytics and the consortium CANCER-ID developing procedures for liquid biopsy analyses for the pharmaceutical industry. Most recently TATAA Biocenter developed Two-Tailed PCR, which is currently the most sensitive and specific method for microRNA analysis.