2023 Scientific Program

Four topics were discussed during the symposium highlighted by keynote lecturers, experts in their field.

• Methodological Approaches & Instruments
• Solid-tissue Analysis & Spatial Omics
• Circulating Biomarkers, EV & Single Cells
• Integrative analysis from Omics to Bioinformatics

Download the complete book of abstracts here.

Final Scientific Program

Thursday, June 29^th, 2023

8:00-9:00 Registration

9:00-9:10 Welcome : Alessandra Petrucci, Rector of the University of Florence

Introduction :

Session 1: New Methodological Approaches and Instruments in Microgenomics Analysis

Chairman: Claudia Bevilacqua & Alexandra Whale

9:10-9:40 Keynote Lecturer - Mikael Kubista (Czech Republic) - Spatiotemporal characterization of wound healing and regeneration in amphibians and of ischemic brain injury in mice

9:40-10:10 Invited Lecturer - Davide Cittaro (Italy) - Sketching chromatin dynamics at single cell level

10:10-10:40 Invited Lecturer - Viacheslav MYLKA (Belgium) – Doing more with less: comparative analysis of sample multiplexing methods for single-cell RNA-seq

10:40-10:55 Selected oral presentation - Nikoletta Galambos (France) - Young Scientist Grant - Cellular specialization inside the symbiotic organ of an insect pest: deciphering the dialogue between host and symbiotic bacteria and their adaptation to different dietetic conditions

10:55-11:25 : Coffee break + Posters + Sponsors

11:25-11:55 Invited Lecturer - Alexandra Whale (UK) - Using digital PCR to characterise viral vector genomes

11:55-12:15 GOLD sponsor presentation - BIO-RAD - Sergey Yakushev (Switzerland) - Examples of the duplex and multiplex ddPCR applications from the contract research organization practice

12:15-12:30 Selected oral presentation - Eloise Debare & Benjamin Tisserand (France) - OPTO-Cas9 allows easy, fast and precise spatiotemporal genome editing in vertebrate embryo at cellular resolution

12:30-12:45 Selected oral presentation - Adele Calabri (Italy) - Monitoring circulating tumor DNA during immunotherapy in Non-Small Cell Lung Cancer patients by targeted NGS: preliminary data on the CORELAB project

12:45-13:00 Invited oral presentation : Fabio Morecchiato (Italy) – dPCR vs. qRT-PCR for quantitative detection of SARS-CoV-2 RNA in environmental specimens

13:00-13:45 : Lunch + Posters + Sponsors

13:45-14:15 :  Bio-Rad Lunch Conference

Session 2: Solid Tissues and Spatial Omics

Chairman: Pamela Pinzani & Mikael Kubista

14:30-15:00 Keynote Lecturer: Pascal Barbry (France) - Omics at the bedside of human lung diseases

15:00-15:30 Invited Lecturer: Evren Azeloglu (USA) - Integrated multiomics for creation of a human kidney tissue atlas

15:30-15:45 Selected oral presentation: Giorgia Egidy (France) - Spatiotemporal analysis of melanoma heterogeneity in a spontaneous swine model at single-cell resolution

15:45-16:15 Coffee break + Posters + Sponsors

16:15-16:45  Invited Lecturer: Letizia De Chiara (Italy) - Single Cell RNA sequencing to identify rare populations in complex organs

16:45-17:00 Selected oral presentation: Lorenzo Giordani (France) - Multimodal Spatial profiling of Neuromuscular Disorders

17:00-17:30 Invited Lecturer : Ying Zhu (USA) - Deep spatial proteomics at cellular and subcellular resolution for human and plant biology

17:30-17:45 Selected oral presentation: Maria Elena Melica (Italy) - Single-cell enable the identification of the molecular mechanisms driving drug-dependent restoration of the filtration barrier in crescentic glomerulonephritis

20:00 Gala dinner at Hotel Baglioni, Piazza dell'Unita Italiana 6 - 50123 Firenze

Friday, June 30^th, 2023

Session 3: Circulating Biomarkers & Extracellular Vesicles & Single-Cells

Chairman: Michael Pfaffl & Patrice Martin

8:30-9:00 Keynote Lecturer: Pieter Mestdagh (Belgium): Cell free RNA biomarkers in cancer

9:00-9:30 Invited Lecturer: Simon Carding (UK) - Bacterial extracellular vesicle; Spheres of influence within and beyond the gut

9:30-10:00 Invited Lecturer: Michael Pfaffl (Germany) - A development and analysis pipeline for microRNA biomarker signatures in molecular diagnostics based on circulating EV

10:00-10:15 Selected oral presentation: Matteo Cuccato (Italy) - Extracellular vesicles miRNome profiling for subclinical mastatis detection

10:15-10:45: Coffee break + Posters + Speakers

10:45-11:00 Selected oral presentation: Davide Ferraro (Italy) - Fast and reproducible method for extracellular vesicle isolation by novel droplet microfluidics device

11:00-11:30 Invited Lecturer: Christian Preusser (Germany) - Using single-cell sequencing and Cre-Lox reporters for tracking tumor EV

11:30-11:45 Selected oral presentaation: Giulia Cantini (Italy) - Circulating tumor cells: a novel technique for single cell isolation and analysis in adrenocortical carcinoma

11:45-12:05 2^nd GOLD sponsor presentation: QIAGEN - Monique van Eijndhoven & Cristina Gomez (The Netherlands) - Single-nucleotide resolution sequencing (IsoSeek) of plasma EV-associated miRNAs for treatment response prediction in Multiple Myeloma patients

12:05-12:45: Lunch + Posters + Sponsors

12:45-13:15: QIAGEN Lunch Conference

Session 4: New Approaches and Instruments for Microgenomics Analysis

Chairman: Alexandra Whale & Daan Noordemeer

14:00-14:30 Keynote lecturer: Daan Noordermeer (France) - Allele-specific multi-omics investigation of imprinted gene domains using phased long-reads

14:30-15:00 Invited Lecturer: Benedikt Kirchner (Germany) - Hidden layers of complexity - How small RNA distribution and sample handling affect sequencing results

15:00-15:30 Keynote Lecturer: Chiara Lanzuolo (Italy) - SAMMY-SEQ: A new technology to capture Dysfunctional Chromatin Landscapes

15:30-15:45 Selected oral presentation: Adrien Dufour (France) - Multiomics analysis of pig pre-implantation embryo

15:45-16:15 Invited Lecturer: Stephen Clark (UK) - Single-cell multiomicssequencing to investigate the role of the epigenome in cell fate decisions during mouse embryogenesis

16:15-16:30: Closing remarks