Complete book of abstracts : download here.

June 28

Workshop 1 : Methods & Strategies for Molecular Biomarker Analyses

Par Mikael Kubista

• Background and history
• qPCR - how to plan, optimize and validate
• dPCR - how to plan, optimize and validate
• NGS - how to plan, optimize and validate
• Protein analysis with NGS (profiling) and dOCR (ultrasensitive targeted)
• Preanalytics - How to identify the confounding step and how to optimize
• The MIQE and dMIQE guidelines, ISO guidelines
• Discussion and Q&A

Workshop 2 : Spatial Transcriptomics & Single Cell

• Single cell analysis: an overview, Pamela Pinzani, University of Florence, Italy.
• Single cell RNA sequencing and applications, Letizia De Chiara, University of Florence, Italy.
• Single Cell 3.0: From Bench to Insight, Parse Biosciences.
• Spatial biology: an overview, Lance A. Liotta, Center for Applied Proteomics and Molecular Medicine, George Mason University, USA.
• Laser microdissection: advantages and challenges for single cell applications, Lance A. Liotta, Center for Applied Proteomics and Molecular Medicine, George Mason University, USA.
• Targeted Spatial Molecular profiling for Precision Medicine. Molecular and Spatial Profiling of Individual cells, Lance A. Liotta, Center for Applied Proteomics and Molecular Medicine, George Mason University, USA.
• Finding “The One”: How microdissection and cell picking uncovers the hidden world of single cells, Jörg Brühmann, Molecular Machines & Industries (MMI)
• Explore new dimensions through spatial context, Vizgen (Spatial Transcriptomics).
• From multitude to singularity: An up-to-date overview of scRNA-seq data generation and analysis, Roberto Semeraro, University of Florence, Italy.

Sponsored by :

Workshop 3 : Extracellular Vesicles

• Introduction & MISEV guidelines, Michael Pfaffl (moderation), TUM, School of Life Sciences, Freising, Germany.
• Extracellular Vesicles – How to find the Needle in the Haystack, Christian Preusser, EV Core Facility, Medical Faculty, University Marburg, Germany.
• Convenient and Robust Isolation of EV RNA and Intact EVs, Martin Schlumpberger, Qiagen R&D, Hilden, Germany.
• Next Generation of NTA – Fluorescence Colocalisation Measurements, Christina Klasen, Particle Metrix R&D, Inning am Ammersee, Germany.
• EV Biomarker Discovery - Comparison of EV Isolation Methods, small-RNA Seq and RT-qPCR validation, Michael Pfaffl, TUM, School of Life Sciences, Freising, Germany.
• Digital PCR-Based Detection and Characterization of Extracellular Vesicle Biomarkers, Afif Abdel Nour & Ellen Bruske, Qiagen R&D, Hilden, Germany.
• caRNAge -- comprehensive small RNA Seq data analysis, Benedikt Kirchner, TUM, School of Life Sciences, Freising, Germany.

Sponsored by :

Workshop 4: Digital PCR & Microgenomics

• Principles of dPCR & dMIQE, Alexandra Whale, Science Leader, Molecular Biology, National Measurement Laboratory at LGC, UK
• Bio-Rad Droplet Digital™ PCR : for a multiplex of reasons, Lara Rossini, Regional Sales Specialist for Genomic - Southern Europe, BioRAD,
• Use of dPCR in GMO diagnostics, Alexandra Bogožalec Košir, Scientific Associate, National Institute of Biology, Department of Biotechnology and Systems Biology, Slovenia
• Digital PCR improves analysis of circulating cell-free miRNA in prostate cancer patients, Guerit Theil, Head of the Laboratory, Department of Urology and Transplantation, Martin Luther-Univsersität Halle-Wittenberg, Denmark.
• Improving Gene Research Applications with Quant Studio Absolute Q dPCR - Monica Boita, Senior Genetic Analysis Sales Specialist, Thermo Fisher Scientific.
• Data analysis and advanced assay design, Alexandra Whale, National Measurement Laboratory at LGC, UK.

Sponsored by: